Abstract:Amphibians are considered the most threatened vertebrate group in the world, and their population status is not optimistic. It is extremely important to clarify the current status of regional amphibian resources and species distribution. It is not only the basis for carrying out regional protection and research, but also an important basis for formulating corresponding protection strategies. In order to better serve the biodiversity conservation in western Hubei, based on the existing literatures, the checklists of amphibians in the three National Nature Reserves of Wudaoxia, Houhe and Shennongjia were reorganized and revised. The results confirmed that there are 48 amphibian species (belonging to 26 genera, 11 families, and 2 orders) in the three National Nature Reserves. Wudaoxia National Nature Reserve contains 2 orders, 9 families, 18 genera and 25 species of amphibians. Houhe National Nature Reserve contains 2 orders, 10 families, 19 genera and 30 species of amphibians. Shennongjia National Nature Reserve contains 2 orders, 10 families, 21 genera and 35 species of amphibians. There are 6 distribution types of amphibians in Wudaoxia and Shennongjia, and 5 types in Houhe, all of which are dominated by South China type (S). The F index, G index and G-F index of Shennongjia Nature Reserve are the highest, indicating that the diversity of amphibians in Shennongjia Nature Reserve is high at the family and genus levels. Similarity analysis shows that the amphibian communities in Wudaoxia and Shennongjia have the highest similarity. The distribution pattern of amphibian species is affected by multiple environmental factors or by multiple hypotheses. The study provides basic data for the later research and protection of amphibians, as well as the optimization and adjustment of Natural Reserves in western Hubei Province.