湖北林业科技

Miscanthus lutarioriparius, a pioneer species for ecological restoration, demonstrates dual functionality in soil-water conservation and flood adaptation mechanisms. This study employed vegetative organs of M. lutarioriparius obtained from riparian floodplain populations in the Jianghan Floodplain along the Yangtze River as experimental materials. We employed manua sectioning under stereo-microscopic guidance combined with histochemical localization staining and integrated brightfield/fluorescence microscopy imaging to systematically characterize the anatomical structures and apoplastic barriers of adventitious roots, stems, and leaves in M. lutarioriparius. The results showed that: （1）M. lutarioriparius possesses adaptive traits for Jianghan Floodplain environments, including lysigenous aerenchyma (root-stem-leaves) and multilayered apoplastic barriers (suberized/lignified endodermis/exodermis, stele, sclerenchyma tissues, and vascular systems in the vegetative organs). （2）M. lutarioriparius adventitious roots, stems, and leaves feature suberized steles and vascular conduits critical for material transport, with leaves displaying a typical C4 plant Kranz anatomy (dual-layered bundle sheath complexes of sclerenchymatous/parenchymatous cells). （3）M. lutarioriparius employs apoplastic barrier structures, synergistically integrated with vascular tissues and mechanical tissues, to encapsulate its aerenchyma. Under submergence stress, this structural coupling coordinately sustains gas exchange, nutrient transport, and structural integrity, thereby ensuring aerobic metabolism and morphogenesis. （4）The vegetative mass of M. lutarioriparius has lignin and suberin components and has the potential as a bioenergy crop. The anatomical structure and histochemical specialization of?M. lutarioriparius?not only facilitates adaptation to seasonal flood environments in the Jianghan Floodplain but also provides theoretical foundations and germplasm selection criteria for the ecological restoration projects in the Yangtze River Basin.