Abstract:Comprehensive assessment of the diversity and distribution characteristics of nationally protected wild plant species within the Hubei Qizimei Mountain National Nature Reserve was conducted through a two-year grid-based survey. The results indicated that (1) a total of 59 national protected wild plant species were recorded within the Hubei Qizimei Mountain National Nature Reserve, belonging to 26 families and 40 genera. Among these, 3 species were classified as Class I protected species, and 56 as Class II protected species. The Orchidaceae and Liliaceae were dominant with a higher number of protected species in the reserve; (2) seven species of the Paris genus were distributed in the reserve, accounting for 31.82% of the known national distribution, highlighting the genus as a dominant and characteristic group in this reserve; (3) Davidia involucrata and Fagus hayatae were dominant species with larger plant populations and greater numbers in this reserve; (4) thirty-five protected species had only one distribution point in the reserve, indicating a need to strengthen conservation and reintroduction efforts through artificial breeding; (5) the distribution of national protected wild plants was mainly concentrated in the Qizimei Mountain area at altitudes of 800~<1600m within the reserve. The findings provided a scientific basis for the management of protected species, public education, and scientific research in the Hubei Qizimei Mountain National Nature Reserve.