Abstract:The leaf epidermis cells of wilderness Cynodon dactylon which grew in 3 different environments were investigated. The appearance indexes of their leaves, such as the number of stoma, stoma density, the length and width of long cell, short cell and stoma were measured. The results showed that the size order of long cell of the leaf epidermis in Cynodon dactylon were sunny dry land, sunny ground and shady dry land. The size of leaf epidemis long cell demonstrated remarkable difference(p<0.05). The size of the short cell in sunny ground and sunny non-irrigated farmland or shady non-irrigated field was significant(p<0.05). But the difference in sunny filed and shady non-irrigated ground was not remarkable. The ratio of length to width of the stoma showed remarkable difference(p<0.05). The order of ratio was sunny non-irrigated field, shady non-irrigated ground, shady non-irrigated. The stoma density of Cynodon dactylon leaf epidermis in three different habitats demonstrated significant difference (p<0.05). The sunny field, the sunny non-irrigated field, the shady non-irrigated field, which were 319.3, 278.22, 240.01个·mm?2 separately.