湖北林业科技

Through the study on the tissue culture technology of the new Cymbidium sinense variety ‘Mengzhilan’, We explored the effects of different hormone concentrations on the induction initiation, subculture, rooting and seedling culture and different matrix ratios of ‘Mengzhilan’ on the survival rate of transplanting. The results showed that the optimal medium for the induction to ‘Mengzhilan’ was Modified MS+6BA1.5 mg·L-1+KT0.6 mg·L-1, and the best subculture medium was Modified MS+6BA1.5 mg·L-1+KT0.4 mg·L-1+NAA0.6 mg·L-1, the best rooting medium is 1/2 Modified MS+IBA1.0 mg·L-1, The best transplanting substrate is pine bark∶perlite=1∶1.