Abstract:The drought tolerance of Woodwardia japonica, Neolepisorus ovatus and Coniogramme japonica from Zhejiang Province was tested in this paper. The three kinds of plants were put in greenhouse under the condition of drought stress in a natural way. Physiological and biochemical parameters and leaf water retention ability were measured every five days. The results indicated that the variation of water retention capacity in the leaves of Woodwardia japonica was the most significant, the next was Coniogramme japonica, and the slightest was Neolepisorus ovatus. Compared with control, the relative electric conductivity of groups were persistently increased. The chlorophyll content of all of three plants decreased, and that of Coniogramme japonica decreased the most. The trend of activity of POD, SOD, the content of MDA and soluble protein rose first and felled finally with drought stress turning severe. In detail, the activity of POD and SOD and MDA content of Coniogramme japonica increased rapidly in early time, while the Neolepisorus ovatus and Woodwardia japonicaincreased slowly. Comprehensive analysis showed that the order of the drought-tolerance ability of three plants is Woodwardia japonica>Neolepisorus ovatus>Coniogramme japonica.