湖北林业科技

Using tissue culturing of young stem nodes with axillary buds, the rapid propagation system of Populus lasiocarpa was established. The results showed that the explants growing on pure MS medium had a germination rate of 31.3%. The optimal medium for axillary bud induction was MS+NAA0.1 mg/L+6-BA0.1 mg/L, and on which the germination rate was 100% at the 30th day after inoculation. The optimal medium for proliferation culture was MS+NAA0.1 mg/L+6-BA2.0 mg/L, and on which the multiplication index was 4.0 and the shoots were strong at the 30th day after inoculation. The optimal medium for rooting was 1/2MS+NAA0.1 mg/L+IBA0.2 mg/L, and on which the rooting rate was 100% and the average root number was 4 and the average root length was 4.7 cm at the 30th day after inoculation.