Abstract:Abstract: The authors explored suitable technical points of industrial production by tissue culture and short-term cultivation of Aoectochilus formosanus. the most suitable induced medium is MS+6-BA1.0 mg.L-1+KT0.3 mg.L-1+NAA0.3 mg.L-1+sucrose30g.L-1+ carrageenan8g.L-1;the most suitable proliferation medium is MS+6-BA1.0 mg.L-1+KT0.5 mg.L-1+NAA0.3 mg.L-1 +sucrose30g.L-1+ carrageenan8g.L-1;the most suitable strong seedling and rooting medium is MS+IBA0.5 mg.L-1+NAA0.3 mg.L-1 + sucrose20 g.L-1+ carrageenan8g.L-1;ph value 5.8;the most transplantation matrix is peat soil 50%+sand 20%,and management of water, fertilizer,air,heat for industrial production of Aoectochilus formosanus.