湖北林业科技

In this research, observation was carried on the plant morphology of somatic hybrid between Microcitrus Papuana and Rough Lemon, and determination and analysis were carried on the pollen fertility of somatic hybrid .The main results of this research were as follows: 1). Regenerative plant has strong growth potential, high tree body, similar plant morphology with Rough Lemon, larger different morphology from Microcitrus Papuana and wide range of leaf thickness that are very close to Microcitrus Papuana . 2). Pollen vigor of somatic hybrid is normally between 50% and 90.00%. 3). Pollen fertility of somatic hybrid is between that from both parents, but tends to that from parent with high value of pollen vigor. This test shows that gene recombination of M.papuana and Rough Lemon in somatic hybrid between M.papuana and Rough Lemon via protoplast fusion was obtained. But, the fertility of somatic hybrid is stable and can serve as hybrid parent material for future utilization.